Premium
Characterization of p53 gene mutations in basal‐cell carcinomas: Comparison between sun‐exposed and less‐exposed skin areas
Author(s) -
Matsumura Yasuhiro,
Nishigori Chikako,
Yagi Takashi,
Imamura Sadao,
Takebe Hiraku
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960315)65:6<778::aid-ijc12>3.0.co;2-0
Subject(s) - carcinogenesis , basal cell carcinoma , biology , gene , polymerase chain reaction , mutation , genetics , microbiology and biotechnology , dna , basal cell , pathology , medicine
Mutations in the p53 gene in 32 basal‐cell carcinomas (BCC) developed in Japanese patients were identified by the polymerase chain reaction and single‐strand‐conformation polymorphism analysis, followed by sequencing the DNA. Among 16 BCC developed in continuously sun‐exposed areas, 6 tumors showed 7 base substitutions, most of which were G:C to A:T transitions, mainly at the dipyrimidine sites. Seven out of 16 BCC developed in less‐exposed areas showed 8 base substitutions, but the majority (75.0%) of them were transversions. These results suggest that the mutation in the p53 gene plays a significant role in the tumorigenesis of BCC developed in less‐exposed areas as well as those in sun‐exposed areas in Japanese patients. There must be therefore causative factors other than UV irradiation for BCC in less‐exposed areas. © 1996 Wiley‐Liss, Inc.