Premium
Biliary glycoprotein (BGP) expression on T cells and on a natural‐killer‐cell sub‐population
Author(s) -
Möller Marcus J.,
Kammerer Robert,
Grunert Fritz,
von Kleist Sabine
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960315)65:6<740::aid-ijc5>3.0.co;2-z
Subject(s) - biology , cytotoxic t cell , antigen , cd16 , population , microbiology and biotechnology , natural killer cell , interleukin 21 , immunology , cancer research , in vitro , medicine , cd3 , cd8 , biochemistry , environmental health
Human T and natural‐killer (NK) cells, that are thought to be the major cytotoxic effector‐cell populations in the defence against neoplastic cells, were isolated from blood and decidua in order to analyze their expression of carcinoembronic‐antigen‐(CEA)‐family‐member proteins. Biliary glycoprotein (BGP, CD66a) was the only member of the carcinoembryonic antigen family detected. While freshly isolated T‐cells expressed low amounts of BGP, freshly isolated NK cells were negative. After in vitro stimulation for 3 days,T cells up‐regulated their BGP expression and a sub‐group of NK cells (CD16− CD56+), known to predominate in decidua, revealed de novo expression of BGP. In contrast, stimulated CD16+ CD56+ NK cells, which occur exclusively in the blood, remained negative. The expression of BGP could be shown on the protein level by using a panel of 12 well‐defined MAbs and on the transcription level in rt‐PCR and subsequent oligonucleotide hybridization. Interestingly, rIL‐2‐stimulated T cells expressed 3‐fold higher levels of BGP compared with those seen after stimulation with phytohemaglutinine (PHA). PHA, on the other hand, induced a higher expression of HLA‐DR, an activation marker of T cells. The differential regulation implies a distinct function of BGP and HLA‐DR. © 1996 Wiley‐Liss, Inc.