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Modulation of MR‐visible mobile lipid levels by cell culture conditions and correlations with chemotactic response
Author(s) -
Delikatny Edward J.,
Lander Catherine M.,
Jeitner Thomas M.,
Hancock Rebecca,
Mountford Carolyn E.
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960117)65:2<238::aid-ijc18>3.0.co;2-9
Subject(s) - lipid metabolism , cell culture , chemotaxis , phosphocholine , choline , catabolism , biology , biochemistry , phospholipid , lipid signaling , chemistry , biophysics , microbiology and biotechnology , metabolism , membrane , phosphatidylcholine , enzyme , receptor , genetics
A transformed murine fibroblast cell line has been used to assess which criteria govern the appearance of a lipid pool that is mobile on the MR time scale. A high‐resolution proton MR signal arising from neutral lipids, including triglyceride and cholesteryl esters, has previously been associated with membrane events in stimulated, transformed and malignant cells. We report that the attenuation of cellular proliferation by confluence or low pH caused significant increases in MR‐visible lipid and that the lipid signal could be amplified at high density by the removal of serum. A significant decrease in chemotactic response accompanied the culture of cells at high density, but chemotactic response was not generally linked to alteration of the lipid signal. The appearance of the signal was also not correlated with the proportion of cells in any phase of the cell cycle. Significant changes in the MR‐visible pools of the lipid metabolites choline, phosphocholine and glycerophosphocholine were measured under the culture conditions employed with 2D MRS and suggest that MR‐visible lipid may arise from the catabolism of phospholipids. © 1996 Wiley‐Liss, Inc.