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Preferred nucleotide sequence at the integration target site of human T‐cell leukemia virus type I from patients with adult T‐cell leukemia
Author(s) -
Chou Kuan S.,
Okayama Akihiko,
Su IhJen,
Lee TunHou,
Essex Max
Publication year - 1996
Publication title -
international journal of cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.475
H-Index - 234
eISSN - 1097-0215
pISSN - 0020-7136
DOI - 10.1002/(sici)1097-0215(19960103)65:1<20::aid-ijc4>3.0.co;2-3
Subject(s) - provirus , leukemia , biology , virology , t cell leukemia , nucleic acid sequence , virus , adult t cell leukemia/lymphoma , nucleotide , lymphoma , human t lymphotropic virus 1 , genetics , dna , genome , microbiology and biotechnology , immunology , gene
Human T‐cell leukemia virus type I (HTLV‐I) is etiologically associated with adult T‐cell leukemia/lymphoma (ATL). We cloned and sequenced host DNA adjacent to the long terminal repeats of HTLV‐I from uncultured leukemic cells of 4 ATL patients. The region flanking the provirus was generally A/T‐rich (60–64% A/T), and a nucleotide composition bias was noticed when sequences within 25 bp on both sides of the integration target site were analyzed. In the 6‐bp direct repeat, both end positions are preferentially occupied by G/C, whereas the middle positions are preferentially occupied by A/T. Furthermore, AA or TT dinucleotides are frequently present on each side adjacent to the center of the direct repeat. Our finding suggests preferential integration target sites of HTLV‐I in the host genome. Further study is warranted to determine whether each of the target sequence preference is a general property of HTLV‐I integration or may be associated with the leukemogenesis of ATL. © 1996 Wiley‐Liss, Inc.