Open AccessSelective modulation of specific protein kinase C (PKC) isoforms in primary human megakaryocytic vs. erythroid cells
Author(s) -
Marchisio Marco,
Bertagnolo Valeria,
Celeghini Claudio,
Vitale Marco,
Capitani Silvano,
Zauli Giorgio
Publication year - 1999
Publication title -
the anatomical record
Language(s) - English
Resource type - Journals
eISSN - 1097-0185
pISSN - 0003-276X
DOI - 10.1002/(sici)1097-0185(19990501)255:1<7::aid-ar2>3.0.co;2-t
Subject(s) - haematopoiesis , protein kinase c , gene isoform , cytoplasm , biology , microbiology and biotechnology , megakaryocyte , cd34 , immunofluorescence , progenitor cell , astrocyte , cell culture , kinase , stem cell , biochemistry , immunology , antibody , gene , endocrinology , genetics , central nervous system
We have investigated the pattern of expression of classical (α, βI, βII, γ), novel (δ) and atypical (ζ) protein kinase C (PKC) isoforms during the course of human hematopoietic differentiation along the closely related megakaryocytic and erythroid lineages. Using in situ immunofluorescence analysis, freshly isolated human pluripotent CD34 + hematopoietic progenitor cells expressed detectable amounts of all the PKC isoforms investigated. On the other hand, clear‐cut differences in terms of PKC staining were noticed between cells belonging to the erythroid and megakaryocytic lineages, obtained after 9 days of serum‐free liquid culture in the presence of specific growth factors. Specifically, 1) erythroid cells showed a very weak expression of PKC‐α, ‐βI, ‐βII, and ‐γ, while megakaryocytes showed an enhanced expression of all classical PKC isoforms, predominantly confined to the cytoplasm; 2) the expression of PKC‐δ increased in the cytoplasmic and nuclear compartments of both erythroid and megakaryocytic cells with respect to CD34 + cells; and 3) atypical PKC‐ζ isoform showed a striking accumulation in the nucleus during both erythroid and megakaryocytic differentiation. Anat Rec 255:7–14, 1999. © 1999 Wiley‐Liss, Inc.