Open AccessLineage‐related susceptibility of human hemopoietic cell lines to apoptosis
Author(s) -
Mariani Adriana Rita,
Columbaro Marta,
Zauli Giorgio,
Zamai Loris,
Luchetti Francesca,
Gobbi Pietro,
Ghibellini Dora,
Falcieri Elisabetta,
Vitale Marco
Publication year - 1999
Publication title -
the anatomical record
Language(s) - English
Resource type - Journals
eISSN - 1097-0185
pISSN - 0003-276X
DOI - 10.1002/(sici)1097-0185(19990101)254:1<1::aid-ar1>3.0.co;2-6
Subject(s) - dna laddering , apoptosis , jurkat cells , haematopoiesis , biology , flow cytometry , k562 cells , lineage (genetic) , microbiology and biotechnology , myeloid , cell culture , lineage markers , immunology , dna fragmentation , programmed cell death , genetics , t cell , immune system , stem cell , progenitor cell , gene
Apoptosis plays a fundamental role in shaping normal hematopoiesis. We have investigated the relationship existing between susceptibility to apoptosis and lineage commitment in hemopoietic cells. The presence and degree of apoptosis were investigated in myeloid (HL‐60 and K562), T (Jurkat and MOLT‐4), and B (CESS and Raji) lymphoid cell lines by using a variety of techniques—transmission electron and light microscopy, flow cytometry and DNA gel electrophoresis. The major achievement of this study is that hematopoietic cells respond to different chemical (staurosporin, tiazofurin, camptothecin) and physical (hyperthermia or hypothermia) stimuli by apoptosis in a lineage‐related way. Moreover, with respect to the methods used to detect apoptosis, a strong correlation was observed between the presence of the hypodiploid peak determined by flow cytometry and the DNA laddering evaluated by gel electrophoresis, but both techniques failed to demonstrate the presence of apoptosis in some cases. We conclude that cells of different hematopoietic lineages mostly show a lineage‐related behaviour in their apoptotic response to different stimuli, suggesting that the lineage commitment and the stage of differentiation can confer different sensitivities to specific apoptotic stimuli. Moreover, morphological techniques still represent the most reliable approach to detect apoptosis in hemopoietic cells. Anat Rec 254:1–6, 1999. © 1999 Wiley‐Liss, Inc.