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Col2‐GFP reporter marks chondrocyte lineage and chondrogenesis during mouse skeletal development
Author(s) -
Grant T. Dawn,
Cho Jay,
Ariail Kiley S.,
Weksler Nicole B.,
Smith Randall W.,
Horton William A.
Publication year - 2000
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(200006)218:2<394::aid-dvdy12>3.0.co;2-i
Subject(s) - chondrogenesis , chondrocyte , biology , microbiology and biotechnology , green fluorescent protein , cell sorting , sox9 , cartilage , transgene , cell , mesenchymal stem cell , anatomy , gene expression , genetics , gene
Mice were generated in which a Col2‐GFP transgene serves as a reporter for the chondrocyte lineage and for chondrogenesis in live embryos and newborn pups. Cells actively engaged in chondrogenesis were identified by confocal optical sectioning within their native environments in embryos and in thick tissue slices. Chondrocytes exhibiting GFP fluorescence were purified from rib cages by high‐speed cell sorting of crude cell suspensions. Intensity of fluorescence correlated with biosynthesis of procollagen II in these cells. The use of these mice and their cells provides a novel approach for studying chondrocyte differentiation and chondrogenesis during skeletal development. Dev Dyn;218:394–400. © 2000 Wiley‐Liss, Inc.