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YAC transgene‐mediated olfactory receptor gene choice
Author(s) -
Ebrahimi Farah A.W.,
Edmondson James,
Rothstein Rodney,
Chess Andrew
Publication year - 2000
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(200002)217:2<225::aid-dvdy9>3.0.co;2-3
Subject(s) - biology , olfactory receptor , vomeronasal organ , olfactory system , genetics , locus (genetics) , transgene , gene , allele , allelic exclusion , olfactory bulb , receptor , neuroscience , central nervous system , t cell receptor , immune system , t cell
In the mouse, individual olfactory neurons express one of a thousand distinct olfactory receptor genes. Furthermore, only one allele of the expressed gene is transcribed. This phenomenon, random allelic inactivation, along with the observation that the olfactory receptor genes reside in large chromosomal arrays, suggests a role for long‐range gene regulation in olfactory receptor gene choice. We have constructed a 300‐kb yeast artificial chromosome (YAC) transgene in which a single receptor gene is marked while maintaining its coding region. This 300‐kb piece of DNA functions as an independent olfactory receptor gene locus in directing olfactory receptor gene choice in both the olfactory system and the accessory olfactory system (vomeronasal organ, VNO). Furthermore, the transgene, like endogenous olfactory receptor loci, is subject to allelic inactivation. Dev Dyn;217:225–231. © 2000 Wiley‐Liss, Inc.