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RARβ mediates the response of Hoxd4 and Hoxb4 to exogenous retinoic acid
Author(s) -
Folberg Adriana,
Nagy Kovács Erzsébet,
Luo Jiangming,
Giguère Vincent,
Featherstone Mark S.
Publication year - 1999
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(199906)215:2<96::aid-dvdy2>3.0.co;2-t
Subject(s) - retinoic acid , biology , rhombomere , hindbrain , hox gene , retinoic acid inducible orphan g protein coupled receptor , retinoic acid receptor , retinoic acid receptor beta , microbiology and biotechnology , retinoic acid receptor gamma , tretinoin , embryonic stem cell , homeobox , morphogen , genetics , embryo , endocrinology , gene , gene expression
Abstract One action of retinoids in development is the regulation of Hox gene expression. Hoxd4 and Hoxb4 expression in the embryonic hindbrain is anteriorized by retinoic acid (RA) treatment of mid‐gestation mouse embryos. Here we demonstrate that retinoic acid receptor beta ( Rarb ) deficient mice present only a partial anteriorization of either Hoxd4 or Hoxb4 in response to RA treatment. Our results strongly suggest that RARβ is a mediator of their RA‐response, and reveal anteroposterior polarity within a single rhombomere (r). Additionally, we generated mice doubly mutated for Hoxd4 and Rarb in an attempt to identify common morphogenetic pathways between these two genes. We conclude that there are no synergistic interactions between Hoxd4 and Rarb in the specification of the cervical vertebrae. Dev Dyn 1999;215:96–107 . © 1999 Wiley‐Liss, Inc.