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Bmp‐2 downstream targets in mesenchymal development identified by subtractive cloning from recombinant mesenchymal progenitors (C3H10T½)
Author(s) -
Bächner Dietmar,
Ahrens Marion,
Schröder Dietmar,
Hoffmann Andrea,
Lauber Jörg,
Betat Nicole,
Steinert Peter,
Flohé Leopold,
Gross Gerhard
Publication year - 1998
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(199812)213:4<398::aid-aja5>3.0.co;2-t
Subject(s) - biology , mesenchymal stem cell , chondrogenesis , microbiology and biotechnology , adipogenesis , progenitor cell , cloning (programming) , embryonic stem cell , genetics , gene , stem cell , computer science , programming language
A Bmp‐dependent in vitro model was used to identify cDNAs during the manifestation of mesenchymal lineages. This model involves the recombinant expression of Bmps (Bmp‐2, Bmp‐4–7) in murine mesenchymal C3H10T½ progenitors, which leads to the differentiation into three lineages: the osteogenic, the chondrogenic and the adipogenic lineage, albeit in varying efficiencies. By subtractive cloning, 21 Bmp‐2–regulated cDNAs from C3H10T½ mesenchymal progenitors were identified; 20 were related to known sequences and 1 was not. During mouse embryonic development, many of these cDNAs are expressed in chondrogenic, osteogenic, and in adipogenic tissues. Novel findings include a G 0 /G 1 switch gene ( G0S2 ), which was demonstrated to be predominantly expressed in adipose tissue during late murine embryonic development. Furthermore, the membrane‐standing glycoprotein autotaxin (ATX) is expressed, at precartilage condensations, joint regions, and during tooth development. An as yet undescribed cDNA, 29A, which encodes a putative secreted factor, is expressed in developing osteo‐/chondrogenic tissues of vertebrae, ribs, tooth, and the limb bud. C3H10T½‐progenitors, therefore, may serve as a legitimate model for the investigation of the Bmp‐mediated events during mesenchymal differentiation. Dev. Dyn. 1998;213:398–411. © 1998 Wiley‐Liss, Inc.