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Early expression of AMH in chicken embryonic gonads precedes testicular SOX9 expression
Author(s) -
Oreal Emmanuelle,
Pieau Claude,
Mattei MarieGenevieve,
Josso Nathalie,
Picard JeanYves,
CarréEusèbe Danièle,
Magre Solange
Publication year - 1998
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(199808)212:4<522::aid-aja5>3.0.co;2-j
Subject(s) - biology , embryonic stem cell , expression (computer science) , microbiology and biotechnology , genetics , gene , computer science , programming language
In mammals, anti‐Müllerian hormone (AMH) is produced by Sertoli cells from the onset of testicular differentiation and by granulosa cells only after birth. SOX9, a transcription factor related to the testis‐determining factor SRY, is expressed in mouse testis 1 day before AMH. To determine the relationship between AMH and SOX9 in birds, we cloned the AMH promoter in search of SOX9 response elements, and we compared the expression of AMH and SOX9 in the gonads of chick embryos using in situ hybridization. Potential SOX response elements were found in the AMH promoter; however, AMH is expressed in both sexes at stage 25, 1 day before the first SOX9 transcripts appear in the male gonads. SOX9 is never expressed in the female. These results do not support the hypothesis that SOX9 could trigger the expression of testicular AMH in the chick but does not exclude a later role in testis development. Dev. Dyn. 1998;212:522–532. © 1998 Wiley‐Liss, Inc.