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Identification of a receptor‐like protein tyrosine phosphatase expressed during Xenopus development
Author(s) -
Yang Claire Q.,
Friesel Robert
Publication year - 1998
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(199807)212:3<403::aid-aja8>3.0.co;2-l
Subject(s) - biology , protein tyrosine phosphatase , identification (biology) , phosphatase , microbiology and biotechnology , receptor , tyrosine , protein phosphatase 2 , genetics , biochemistry , phosphorylation , ecology
To begin to determine the role of receptor‐like tyrosine phosphatases during Xenopus development, we have isolated a cDNA predicted to encode receptor‐like tyrosine phosphatase with significant amino acid sequence identity to mouse and human protein tyrosine phosphatase α (PTPα). Xenopus PTPα (XPTPα) exists as a maternally expressed mRNA that decreases in expression during gastrulation and then maintains a constant lower level of expression through early tadpole stages. In situ hybridization reveals that XPTPα mRNA is expressed throughout the gastrula stage embryo. During subsequent development, XPTPα mRNA becomes restricted in its expression to various regions of the brain and the visceral arches. XPTPα mRNA is also expressed in several adult tissues and in Xenopus XTC cells. Immunoblot analysis demonstrates that XPTPα protein is expressed at relatively uniform levels throughout development. Expression of XPTPα protein in insect cells with a recombinant baculovirus results in a glycosylated polypeptide of 110–130 kDa with intrinsic phosphotyrosine phosphatase activity. The spatial and temporal patterns of expression of XPTPα indicate that it may play multiple roles during early development including development of the brain. Dev. Dyn. 1998;212:403–412. © 1998 Wiley‐Liss, Inc.