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Gene trap integrations expressed in the developing heart: Insertion site affects splicing of the PT1‐ATG vector
Author(s) -
McClive Peter,
Pall Gurman,
Newton Kathryn,
Lee Muriel,
Mullins John,
Forrester Lesley
Publication year - 1998
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(199806)212:2<267::aid-aja11>3.0.co;2-1
Subject(s) - biology , exon , gene , rna splicing , homologous recombination , microbiology and biotechnology , alternative splicing , reporter gene , pair rule gene , fusion gene , gene targeting , chimeric gene , genetics , gene expression , regulator gene , rna
We describe the characterisation of three gene trap integrations in embryonic stem cells in which the lac Z reporter gene is repressed by retinoic acid (RA) in vitro and is expressed in the developing heart in vivo. In one of these, the gene trap vector has integrated into a gene that is located on chromosome 17 and is homologous to the human transcription factor gene, TFEB . Embryonic and adult cardiac expression of both the fusion transcript and the endogenous gene was confirmed. However, we show that the integration has not resulted in a null allele, because wild type transcripts, possibly resulting from splicing around the vector, are observed in homozygous tissue. The other two cardiac‐expressing gene trap integrations have occurred into exons on chromosomes 1 and 5 and have used cryptic donor sites within the vector to generate functional fusion transcripts. One of these exon integrations results in a lethal neonatal phenotype. Dev. Dyn. 1998;212:267–276. © 1998 Wiley‐Liss, Inc.