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Cell‐cell adhesion in limb‐formation, estimated from photographs of cell sorting experiments based on a spatial stochastic model
Author(s) -
Mochizuki Atsushi,
Wada Naoyuki,
Ide Hiroyuki,
Iwasa Yoh
Publication year - 1998
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(199803)211:3<204::aid-aja2>3.0.co;2-l
Subject(s) - cell sorting , biology , cell , cell adhesion , adhesion , biological system , sorting , cell adhesion molecule , biophysics , microbiology and biotechnology , mathematics , algorithm , genetics , chemistry , organic chemistry
We developed a new method to estimate the magnitude of differential cell‐cell adhesion of two tissues based on the spatial patterns in cell‐sorting experiments, and applied it to experimental data on progress‐zone cells of avian limb bud at stages 20–26. The change in cell distribution in the experiment was recorded, and statistics q B/B for the degree of cell sorting was calculated from the photographs. Based on extensive computer simulations of spatial Markov processes on a 2‐D lattice, we derived a formula for q B/B increasing with time. Using least square fitting, differential adhesion A and cell motility m are estimated from the time series data of q B/B obtained from the experiment. The estimated A was close to 0 (the spatial pattern remained random) if the mixed cells were from two tissues of the same stage. If the mixed cells were from different stages, and the estimated A was positive (cell sorting occurred). Estimated A increased with the difference in the stage number of the two tissues from which the cells were sampled. This result can be explained both by the stage‐specific change in adhesion molecules and by a linear increase (or decrease) in the amount of adhesion molecules on cell surface. Dev. Dyn. 1998;211: 204–214. © 1998 Wiley‐Liss, Inc.