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Expression and localization of laminin‐5 subunits during mouse tooth development
Author(s) -
Yoshiba Kunihiko,
Yoshiba Nagako,
Aberdam Daniel,
Meneguzzi Guerrino,
PerrinSchmitt Fabienne,
Stoetzel Corinne,
Ruch Jean Victor,
Lesot Hervé
Publication year - 1998
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(199802)211:2<164::aid-aja5>3.0.co;2-f
Subject(s) - biology , laminin , microbiology and biotechnology , expression (computer science) , protein subunit , anatomy , genetics , extracellular matrix , gene , computer science , programming language
Tooth morphogenesis is regulated by epithelial‐mesenchymal interactions mediated by the basement membrane (BM). Laminins are major glycoprotein components of the BMs, which are involved in several cellular activities. The expression and localization of the α3, β3, and γ2 laminin‐5 subunits have been analyzed by in situ hybridization and immunohistochemistry during mouse molar development. Initially (E12), mRNAs of all subunits were detected in the entire dental epithelium and the corresponding proteins were located in the BM. During cap formation (E13‐14), transcripts for the α3 and γ2 subunits were localized in the outer dental epithelium (ODE), whereas the β3 subunit mRNA was present in the inner dental epithelium (IDE). During the early bell stage (E16), immunoreactivity for all subunits disappeared from the BM along the IDE, although intense signals for β3 mRNA were detectable in cells of the IDE. Subsequently, when the dentinal matrix was secreted by odontoblasts (E18‐19.5), mRNAs of all three subunits were re‐expressed by ameloblasts, and the corresponding proteins were detected in ameloblasts and in the enamel matrix. Tissue recombination experiments demonstrated that when E16 IDE or ODE was associated with E18 dental papilla mesenchyme, immunostaining for all laminin‐5 subunits disappeared from the BM, whereas when cultured with non‐dental limb bud mesenchyme, they remained positive after 48 hr of culture. These results suggest that the temporo‐spatial expression of laminin‐5 subunits in tooth development, which appears to be differentially controlled by the dental mesenchyme, might be related to the enamel organ histo‐morphogenesis and the ameloblast differentiation. Dev. Dyn. 1998;211:164–176. © 1998 Wiley‐Liss, Inc.