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Grg3 , a murine Groucho ‐related gene, is expressed in the developing nervous system and in mesenchyme‐induced epithelial structures
Author(s) -
Leon Cosmo,
Lobe Corrinne G.
Publication year - 1997
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(199701)208:1<11::aid-aja2>3.0.co;2-4
Subject(s) - biology , mesoderm , genetics , mesenchyme , gene , in situ hybridization , microbiology and biotechnology , gene expression , embryonic stem cell , embryo
We have isolated cDNAs representing multiple members of murine groucho homologues, designated Grg for groucho ‐related genes. Among them, Grg3 appears to produce two transcripts. One of the Grg3 transcript contains coding sequence for a complete Groucho protein homologue. The second transcript contains coding sequence for only the two amino‐terminal domains of the Groucho protein, followed by a hydrophobic tail and a stop codon. We analyzed the expression of both transcripts in mouse embryos using RNase protection and in situ hybridization. Expression was detected during cell determination in the nervous system and in somitic mesoderm, overlapping Notch1 expression and adjacent to Mash1, MyoD and Myf5 expression. Thus, the expression pattern of Grg3 suggests a conserved role in the Notch signalling pathway to regulate expression of basic helix‐loop‐helix proteins and cell determination. Grg3 expression was also consistently detected in epithelial structures undergoing mesenchyme induction. Dev Dyn 208:11–24, 1997. © 1997 Wiley‐Liss, Inc.