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Spatial and temporal relationships between Shh, Fgf4 , and Fgf8 gene expression at diverse signalling centers during mouse development
Author(s) -
Bueno David,
Skinner Judith,
Abud Helen,
Heath John K.
Publication year - 1996
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(199611)207:3<291::aid-aja6>3.0.co;2-c
Subject(s) - fgf8 , biology , limb bud , limb development , zone of polarizing activity , gene expression , in situ hybridization , microbiology and biotechnology , gene , apical ectodermal ridge , signalling , sonic hedgehog , regulation of gene expression , genetics , fibroblast growth factor , receptor , mesoderm , embryonic stem cell
During limb outgrowth, Shh, Fgf4 , and Fgf8 act as signals controlling limb growth and patterning. Because these genes are expressed in the limb bud and other known signalling centers, we have explored the relationships between the expression of these genes during mouse development using double in situ hybridization. Within the node and limb bud the expression domains of these genes contact each other, whereas in the floor plate the Fgf8 expression domain does not contact that of Shh . The relative temporal order of gene expression varies in different centers. The spatial and temporal expression of Fgf4, Fgf8 , and Shh suggests the conservation of molecular mechanisms in different organizing centers as well as differences between them. © 1996 Wiley‐Liss, Inc.