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Analysis of MyoD, myogenin, and muscle‐specific gene mRNAs in regenerating Xenopus skeletal muscle
Author(s) -
Nicolas Nathalie,
Gallien Claude L.,
Chanoine Christophe
Publication year - 1996
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(199609)207:1<100::aid-aja9>3.0.co;2-m
Subject(s) - myogenin , myod , myogenesis , biology , myosin , skeletal muscle , xenopus , microbiology and biotechnology , myogenic regulatory factors , cardiotoxin , myod protein , actin , regeneration (biology) , myocyte , gene , genetics , anatomy
We have analyzed in adult Xenopus laevis , using in situ hybridization, the spatial and temporal expression patterns of MyoD, myogenin, and α‐skeletal actin and fast myosin heavy chain mRNAs during muscle regeneration following cardiotoxin injury. MyoD transcripts could be detected in the satellite cells as early as the first stage of regeneration and were expressed persistently throughout the regeneration process. Myogenin mRNAs were transiently expressed in forming myotubes. α‐Skeletal actin and fast myosin heavy chain mRNAs were detected precociously, before the young myotube stage. This work has shown, for the first time, the presence of myogenin transcripts during Xenopus myogenesis. © 1996 Wiley‐Liss, Inc.