Premium
Quantitative analysis of proliferation and cell cycle length during development of the rat retina
Author(s) -
Alexiades Macrene R.,
Cepko Constance
Publication year - 1996
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(199603)205:3<293::aid-aja9>3.0.co;2-d
Subject(s) - biology , retina , cell cycle , retinal , microbiology and biotechnology , mitosis , cell growth , cell , thymidine , cell division , anatomy , neuroscience , genetics , dna , biochemistry
The rat retina has been a useful model system for the study of the development of the centralnervous system (CNS). In order to facilitate future studies on the mechanisms that control retinalgrowth, we have quantified the proliferation of retinal cells and the length of the cell cyclethroughout development. For each day during development, the number of mitotic and postmitoticcells per retina, the proportion of cycling cells, S phase length, and cell cycle length weredetermined through quantification of cell numbers and 3 H‐thymidine labeling. Asretinal development proceeds, the proportion of cycling cells decreases, and cell cycle lengthincreases, in part due to an increase in S phase length. © 1996 Wiley‐Liss, Inc.