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Differential expression of C‐protein isoforms in the developing heart of normal and cardiac lethal mutant axolotls ( Ambystoma mexicanum )
Author(s) -
Ward Simone M.,
Dube Dipak K.,
Fransen Margaret E.,
Lemanski Larry F.
Publication year - 1996
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/(sici)1097-0177(199602)205:2<93::aid-aja1>3.0.co;2-z
Subject(s) - biology , ambystoma mexicanum , mutant , gene isoform , microbiology and biotechnology , zoology , anatomy , medicine , genetics , axolotl , regeneration (biology) , gene
Regulated assembly of contractile proteins into sarcomeric structures, such as A‐ and I‐bands,is still currently being defined. The presence of distinct isoforms of several muscle proteinssuggests a possible mechanism by which myocytes regulate assembly during myofibrillo‐genesis.Of several muscle isoforms located within the A‐band, myosin binding proteins (MyBP) arereported to be involved in the regulation and stabilization of thick filaments during sarcomereassembly. The present confocal study characterizes the expression of one of these myosin bindingproteins, C‐protein (MyBP‐C) in wild‐type and cardiac lethal mutant embryos of the axolotl, Ambystoma mexicanum. C‐protein isoforms are also detected in distinct temporal patternsin whole‐mounted heart tubes and thoracic skeletal muscles. Confocal analysis of axolotl embryosshows both cardiac and skeletal muscles to regulate the expression of C‐protein isoforms over aspecific developmental window. Although the CPRO Axslow isoform is presentduring the initial heartbeat stage, its expression is not retained in the adult heart. C‐proteinisoforms are simultaneously expressed in both cardiac and skeletal muscle during embryogenesis.© 1996 Wiley‐Liss, Inc.