Premium
Expression of the simian virus 40 large tumor antigen (Tag) and formation of Tag‐p53 and Tag‐pRb complexes in human brain tumors
Author(s) -
Zhen HaiNing,
Zhang Xiang,
Bu XingYao,
Zhang ZhiWen,
Huang WenJin,
Zhang Ping,
Liang JingWen,
Wang XiLing
Publication year - 1999
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/(sici)1097-0142(19991115)86:10<2124::aid-cncr34>3.0.co;2-d
Subject(s) - oligodendroglioma , ependymoma , glioma , carcinogenesis , medulloblastoma , astrocytoma , choroid plexus papilloma , brain tumor , pathology , medicine , western blot , antigen , cancer , immunohistochemistry , tumor antigen , cancer research , biology , choroid plexus , central nervous system , immunology , immunotherapy , gene , biochemistry
BACKGROUND The presence of simian virus 40 (SV40) in human brain tumors remains a controversial issue. Even if SV40 does exist in brain tumors, the questions of whether it is associated with brain tumorigenesis and by what mechanisms are unknown. METHODS SV40 large tumor antigen (Tag) was investigated by immunoprecipitation, silver staining, and Western blot analysis in 65 brain tumor cases and 8 cases of normal brain tissue. Tag‐p53 and Tag‐pRb complexes were screened by immunoprecipitation and Western blot analysis in 18 and 15 Tag positive tumor tissues, respectively. RESULTS Tag was found in all 8 cases of ependymoma and 2 cases of choroid plexus papilloma, 90% of pituitary adenoma cases (9 of 10), 73% of astrocytoma cases (11 of 15), 70% of meningioma cases (7 of 10), 50% of glioblastoma multiforme cases (4 of 8), and 33% of medulloblastoma cases (2 of 6). Five oligodendroglioma cases, 1 pineocytoma case, and 8 cases of normal brain tissue were negative for Tag. The Tag‐p53 complex was detected in all 18 Tag positive tumors tested and the Tag‐pRb complex was detected in all 15 Tag positive tumors tested. CONCLUSIONS SV40 Tag not only is expressed in brain tumors; it also can form specific complexes with tumor suppressors p53 and pRb. SV40 is correlated with brain tumorigenesis. The inactivation of p53 and pRb due to the formation of Tag‐p53 and Tag‐pRb complexes possibly is a significant mechanism in the etiopathogenesis of brain tumors. Cancer 1999;86:2124–32. © 1999 American Cancer Society.