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Biologic behavior of and p53 overexpression in multifocal renal cell carcinoma of clear cell type
Author(s) -
Haitel Andrea,
Wiener Helene G.,
Blaschitz Ulrike,
Marberger Michael,
Susani Martin
Publication year - 1999
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/(sici)1097-0142(19990401)85:7<1593::aid-cncr22>3.0.co;2-k
Subject(s) - proliferating cell nuclear antigen , medicine , renal cell carcinoma , clear cell , immunohistochemistry , pathology , lymph node , clear cell renal cell carcinoma , monoclonal antibody , antigen , cancer , cancer research , antibody , immunology
BACKGROUND In the treatment of small renal cell carcinoma (RCC), there is controversy between radical and nephron‐sparing surgical treatment because of the risk of tumor multifocality. The biologic behavior of multifocal RCC compared with that of unifocal RCC is not well investigated, and the relevance of p53 and the proliferation markers MIB‐1 and proliferating cell nuclear antigen (PCNA) to multifocal RCC is not yet established. METHODS In this study, p53 protein overexpression was investigated immunohistochemically in 27 multifocal and 65 unifocal clear cell RCCs using a monoclonal antibody (DO‐1). The nuclear expression of p53 was compared with the expression of PCNA and MIB‐1 (Ki‐67 antigen) and other prognostic factors, including grade and stage. RESULTS Thirty‐three RCCs (35.9%) had p53 positive nuclear staining. MIB‐1 positivity was significantly higher in p53 positive tumors than in p53 negative tumors. PCNA positivity was not different in p53 positive tumors compared with p53 negative tumors. Proliferation marker expression was not associated with tumor focality. p53 overexpression was more often found in unifocal tumors than in multifocal tumors. Intracellular accumulation of the p53 protein was related to tumor grade but not to the T classification of tumor stage. In addition, lymph node involvement was significantly associated with p53 overexpression in tumors of the kidney. Focality did not influence progression free survival. CONCLUSIONS This study demonstrated that there is no difference in the proliferative activity or biologic behavior of multifocal and unifocal tumors. Cancer 1999;85:1593–8. © 1999 American Cancer Society.