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Expression of p21 WAF1/CIP1 in adenocarcinoma of the uterine cervix
Author(s) -
Lu Xin,
Toki Toshihiko,
Konishi Ikuo,
Nikaido Toshio,
Fujii Shingo
Publication year - 1998
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/(sici)1097-0142(19980615)82:12<2409::aid-cncr15>3.0.co;2-t
Subject(s) - medicine , adenocarcinoma , immunohistochemistry , progesterone receptor , estrogen receptor , cervix , cervical cancer , cancer research , oncology , cyclin e , lymph node , cyclin d1 , pathology , cell cycle , cancer , breast cancer
BACKGROUND The purpose of this study was to assess the prognostic significance of the expression of estrogen receptor and cell cycle regulatory gene products in cervical adenocarcinoma. METHODS In 40 cases of adenocarcinoma of the uterine cervix and 10 normal cervices, expression of estrogen receptor and cell cycle regulatory gene products (cyclin E, p16, p21 WAF1/CIP1 , p27, p53, and Ki‐67) was studied using immunohistochemical techniques. The survival of the patients was analyzed in terms of such variables as the expression of these molecules in the tumor and conventional clinicopathologic features, and the Cox proportional hazards model was used to predict the survival of patients with cervical adenocarcinoma. RESULTS Expression of estrogen receptor was consistently observed in normal cervical glands, but in cervical adenocarcinoma it was lost (in 28 cases) or significantly diminished (in 12 cases). Normal cervical glandular cells were usually negative for the cell cycle regulatory gene products, whereas 47.5‐85% of cervical adenocarcinomas were positive for these molecules. When the expression of these molecules was analyzed, significant positive correlations were found between p16 and p27, cyclin E and p27, and cyclin E and p21 WAF1/CIP1 . Univariate survival analysis revealed that the presence of parametrial invasion, the presence of lymph node metastasis, negative staining for p21 WAF1/CIP1 , and a moderately or poorly differentiated tumor all correlated significantly with a poor prognosis. In a stepwise regression analysis, the expression of p21 WAF1/CIP1 and negative pelvic lymph nodes were the best predictors of a favorable prognosis. CONCLUSIONS Expression of p21 WAF1/CIP1 correlated with a favorable prognosis for patients with cervical adenocarcinoma and may serve as a useful marker of survival in cases of this disease. Cancer 1998;82:2409‐2417. © 1998 American Cancer Society.