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Phenotype 2 of deoxyribonuclease I may be Used as a risk factor for gastric carcinoma
Author(s) -
Tsutsumi Souichi,
Asao Takayuki,
Nagamachi Yukio,
Nakajima Tamiko,
Yasuda Toshihiro,
Kishi Koichiro
Publication year - 1998
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/(sici)1097-0142(19980501)82:9<1621::aid-cncr4>3.0.co;2-d
Subject(s) - phenotype , isoelectric focusing , gastric carcinoma , deoxyribonuclease i , carcinoma , biology , deoxyribonuclease , allele , medicine , microbiology and biotechnology , pathology , cancer , genetics , gene , dna , enzyme , biochemistry , base sequence
BACKGROUND Human deoxyribonuclease I (DNase I) (EC3.1.21.1) is one of the candidate nucleases that acts in mammalian cells during apoptosis. Genetic polymorphism of DNase I has been classified into 15 phenotypes controlled by 5 autosomal codominant alleles. The purpose of this study was to determine whether DNase I polymorphism is closely related to the incidence of gastric carcinoma. METHODS The phenotype distribution was examined using urine samples obtained from unrelated Japanese patients with gastric carcinoma (n = 97) and benign gastric diseases (n = 76). Phenotyping was performed using isoelectric focusing electrophoresis in thin polyacrylamide gel and immunoblotting with an antihuman DNase I antibody. RESULTS A close statistical association was found between patients with gastric carcinoma and a high frequency of DNase I phenotype 2. However, there was no significant difference in the phenotype distribution between the group of patients with benign gastric diseases and the controls. CONCLUSIONS The findings of the current study suggest that DNase I phenotype 2 may be a marker for gastric carcinoma. Therefore, compared with other phenotypes, DNase I phenotype 2 may have potential for identifying patients who are at risk of harboring or developing gastric carcinoma. Cancer 1998;82:1621‐5. © 1998 American Cancer Society.