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Flow cytometric analysis of peripheral blood and bone marrow for tumor cells in patients with neuroblastoma
Author(s) -
Komada Yoshihiro,
Zhang XaoLi,
Zhou YanWen,
Inaba Hiroto,
Deguchi Takao,
Azuma Eiichi,
Sakurai Minoru
Publication year - 1998
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/(sici)1097-0142(19980201)82:3<591::aid-cncr23>3.0.co;2-w
Subject(s) - neuroblastoma , bone marrow , flow cytometry , minimal residual disease , pathology , medicine , population , microbiology and biotechnology , cancer research , cell culture , immunology , biology , genetics , environmental health
Abstract BACKGROUND Several sensitive surveillance tests reportedly have been used to detect occult neuroblastoma (NB) cells in peripheral blood (PB) and bone marrow (BM). They may be useful in monitoring minimal residual tumor cells. The authors report the feasibility and clinical usefulness of a sensitive flow cytometric assay that has been newly developed and evaluated to detect NB cells. METHODS Nine NB patients and 15 healthy donors were included in the current study. Primary tumor tissues, BM, and PB were examined for the detection of NB cells using a triple‐color flow cytometric assay. Tumor cells in PB and BM, isolated by fluorescence‐activated cell sorting, were used for morphologic studies and differential polymerase chain reaction analysis of N‐myc gene amplification. RESULTS Neuroblastoma cells consistently showed CD9 + /CD56 + /CD45 ‐ phenotype. Flow cytometric analysis could detect NB cells at a level of 1 per 10 4‐5 cells. The CD9 + /CD56 + /CD45 ‐ cell population was absent in normal PB and BM. This assay identified occult NB cells, which were not detected by conventional cytology, in PB and BM obtained from six patients (one of two with Stage II and all five with advanced disease) at diagnosis. Residual NB cells also were detected in PB and BM during therapy. Neuroblast‐like morphology and N‐myc gene amplification of sorted cells confirmed that CD9 + /CD56 + /CD45 ‐ cells were truly NB cells. CONCLUSIONS A triple‐color flow cytometric assay was a sensitive and specific method to detect occult NB cells in PB and BM. This assay could be an additional component of surveillance testing for NB patients. Cancer 1998;82:591‐9. © 1998 American Cancer Society.