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Fibroblastic colony‐forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma
Author(s) -
Chasseing Norma A.,
Bordenave Raul H.,
Bullorsky Eduardo O.,
Diaz Norma B.,
Stemmelin German R.,
Rumi Lia S.
Publication year - 1997
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/(sici)1097-0142(19971115)80:10<1914::aid-cncr7>3.0.co;2-n
Subject(s) - bone marrow , stromal cell , tumor necrosis factor alpha , radioimmunoassay , prostaglandin e2 , medicine , peripheral blood mononuclear cell , prostaglandin e , fibroblast , cell culture , endocrinology , cancer research , biology , in vitro , biochemistry , genetics
BACKGROUND Although alterations of the bone marrow (BM) fibroblast colony‐forming cells are involved in the development of diverse hematologic disorders, these progenitors still have not been well characterized in patients with solid tumors. METHODS The incidence of fibroblast colony‐forming units (CFU‐F) was evaluated in the cultures of unseparated and fractionated light density BM mononuclear cells (MC) from 25 consecutive untreated lung carcinoma patients (LCP) and 16 normal controls (NC). Unseparated MC also were cultured in the presence of indomethacin (10 ‐6 M). Finally, the authors evaluated the spontaneous production of prostaglandin E2 (PGE2) and tumor necrosis factor‐α (TNF‐α) in culture conditioned mediums of unseparated MC by radioimmunoassay and enzyme‐linked immunoadsorbent assay methodology, respectively. RESULTS A decreased number of CFU‐F was observed in unseparated and fractionated (adherent and nonadherent) light density MC cultures from LCP compared with NC. When unseparated MC of LCP were treated with indomethacin, a slightly increase in the number of CFU‐F was found. Adherent MC (stromal cells) achieved confluence only in 44% of LCP primary cultures compared with 100% of NC. Overproduction of PGE2 and TNF‐α was found in the conditioned mediums of LCP compared with the mean values obtained in NC ( P < 0.05 and P < 0.02, respectively). CONCLUSIONS The lack of confluence and suppression of CFU‐F in BM of LCP may be related to the increase production of PGE2 and TNF‐α. Future investigation will allow the determination of how these modifications influence tumor cell growth and will prove if more alterations of the hematopoietic microenvironment imply a worse prognosis. Cancer 1997; 80:1914‐9. © 1997 American Cancer Society.