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Assessment of microsatellite alterations in young patients with gastric adenocarcinoma
Author(s) -
Seruca Raquel,
SobrinhoSimões Manuel
Publication year - 1997
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/(sici)1097-0142(19971001)80:7<1358::aid-cncr26>3.0.co;2-5
Subject(s) - medicine , microsatellite instability , adenocarcinoma , stomach , cancer , gastric carcinoma , gastroenterology , carcinoma , gastric adenocarcinoma , microsatellite , allele , gene , biochemistry , chemistry
BACKGROUNDGenetic factors are probably important in the development of gastric carcinoma in young patients (younger than 40 years). The authors investigated early onset primary gastric adenocarcinomas for the presence of microsatellite instability, which is a phenotypic marker for the hereditary nonpolyposis colon carcinoma syndrome.METHODSDNA was extracted from archival microdissected carcinoma and corresponding normal tissue from 10 British gastric carcinoma patients age 19 to 39 years at the time of diagnosis. A panel of 12 microsatellite loci were amplified by fluorescent polymerase chain reaction and analyzed using an automated DNA sequencer.RESULTSThere was no evidence of microsatellite instability. In contrast, allelic imbalance was recorded at D3S966, D3S1076, D10S197, D11S904, P53, NM23, and DCC microsatellite loci.CONCLUSIONSThe authors reported ten cases of early onset gastric carcinoma that demonstrated allelic imbalance but no evidence of instability at microsatellite loci. It is unlikely that defective DNA mismatch repair is important in this group of young patients.