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Sulfatides as a predictive factor of lymph node metastasis in patients with colorectal adenocarcinoma
Author(s) -
Morichika Hiroshi,
Hamanaka Yuichiro,
Tai Tadashi,
Ishizuka Ineo
Publication year - 1996
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/(sici)1097-0142(19960701)78:1<43::aid-cncr8>3.0.co;2-i
Subject(s) - immunostaining , medicine , colorectal cancer , pathology , lymph node , adenocarcinoma , stage (stratigraphy) , metastasis , lymph node metastasis , lymph , cancer , immunohistochemistry , biology , paleontology
BACKGROUND Sulfatide is one of the acidic glycolipids that are components of the cellular membrane. It has been reported that sulfatide plays some important roles in cell functions, such as cell adhesion. The aim of this study was to investigate the relationship between sulfatide and the malignant potential of colorectal carcinoma. METHODS Glycolipids were extracted from 22 primary colorectal cancer tumors and 6 adjacent normal mucosa using the Folch method. Qualitative analysis of the sulfatide contents was performed using thin‐layer chromatography (TLC) and TLC immunostaining. Quantitative analysis was performed by densitometric scanning. RESULTS Two bands of sulfatide were observed by TLC immunostaining and were designated as cerebroside sulfated ester (CSE)‐A and CSE‐B. Levels of CSE‐A were found to be significantly decreased whereas those of CSE‐B were significantly increased in cancerous tissue when compared with normal tissue ( P < 0.05). The CSE ratios (CSE‐B/[CSE‐A + CSE‐B]) in the 15 tumors showing lymph node metastasis were higher than in the 7 tumors without lymph node metastasis ( P < 0.05). The CSE ratios in 9 Dukes Stage C tumors and 6 Dukes Stage D tumors were higher than those in 7 Dukes Stage A tumors ( P < 0.02, and P < 0.05, respectively). CONCLUSIONS These data support the conclusion that changes in sulfatide composition may play an important role in lymph node metastasis of colorectal adenocarcinoma. Cancer 1996;78:43‐7.