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Immunocytochemical detection of p53 protein from pancreatic duct brushings in patients with pancreatic carcinoma
Author(s) -
Ishimaru Shouhei,
Itoh Masaki,
Hanada Keiji,
Tsuchida Akira,
Iwao Toshiyasu,
Kajiyama Goro
Publication year - 1996
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/(sici)1097-0142(19960601)77:11<2233::aid-cncr8>3.0.co;2-q
Subject(s) - medicine , immunocytochemistry , pancreatitis , pancreatic duct , pancreas , pathology , cytology , carcinoma , pancreatic disease , pancreatic carcinoma , radiology , pancreatic cancer , cancer
BACKGROUND It is often difficult to distinguish pancreatic carcinoma preoperatively from chronic pancreatitis. Therefore, we have developed a new method of detecting p53 immunoreactivity in cytologic material obtained by endoscopic retrograde pancreatic duct brushing (ERPDB). METHODS Twenty‐eight patients with prominent strictures of the main pancreatic duct demonstrated by pancreatography including 20 ductal cell carcinoma and 8 chronic pancreatitis were studied. The ability to distinguish between these two groups preoperatively by conventional cytologic examination was compared with p53 immunocytochemistry using ERPDB. RESULTS The sensitivity, specificity, and overall accuracy of conventional cytologic examination in distinguishing ductal cell carcinoma from chronic pancreatitis were 60%, 100%, and 71%, respectively. In comparison, the sensitivity, specificity, and overall accuracy of p53 immunocytochemistry in distinguishing were 90%, 100%, and 93%, respectively. The sensitivity of p53 staining of specimens from patients with carcinoma of the body or tail of the pancreas (90%) was the same for those with tumors of the head of the pancreas (90%). CONCLUSIONS These results suggest that p53 immunocytochemistry using ERPDB in conjunction with conventional cytologic examination can help differentiate ductal cell carcinoma from chronic pancreatitis preoperatively. Cancer 1996;77:2233‐9.