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Analysis of numerical aberrations in specific chromosomes by fluorescent in situ hybridization as a diagnostic tool in breast cancer
Author(s) -
Ichikawa Daisuke,
Hashimoto Naoya,
Hoshima Masakazu,
Yamaguchi Toshiharu,
Sawai Kiyoshi,
Nakamura Yusuke,
Takahashi Toshio,
Abe Tatsuo,
Inazawa Johji
Publication year - 1996
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/(sici)1097-0142(19960515)77:10<2064::aid-cncr15>3.0.co;2-t
Subject(s) - fluorescence in situ hybridization , medicine , breast cancer , biopsy , pathology , fine needle aspiration , chromosome 17 (human) , breast carcinoma , ductal carcinoma , cancer , chromosome , radiology , biology , gene , genetics
BACKGROUND Biopsy by fine‐needle aspiration has become a routine technique for the diagnosis of a dominant breast mass. In this study, fluorescent in situ hybridization (FISH) analysis of interphase nuclei allowed the authors to detect genetic aberrations that are difficult to identify by conventional cytology. METHODS To investigate ways of minimizing misdiagnosis of the cytology of breast tumors, and detecting genetic aberrations preoperatively, the authors performed FISH with specimens obtained by fine‐needle aspiration biopsies of 106 primary breast tumors (78 primary breast cancers, 2 phyllodes tumors, and 26 benign breast tumors). Numerical chromosome aberrations were investigated using 3‐color FISH performed with (peri)centromere‐specific probes for chromosomes 1, 11, and 17. RESULTS Sufficient materials for FISH analysis were obtained with aspiration biopsy from 98 of the 106 breast tumors (93.4%). None of the benign tumors nor phyllodes tumors showed evidence of aneusomy for any of the 3 chromosomes. However, 71 of the 74 breast cancers (95.9%) for which sufficient material was available demonstrated aneusomy of at least 1 of the 3 chromosomes tested. The FISH analysis also suggested a possible correlation between aneusomy of chromosome 17 and metastasis to regional lymph nodes (chi‐square test = 7.78; P < 0.05). CONCLUSIONS FISH analysis of fine‐needle aspiration biopsies can be a practical and useful method for the preoperative diagnosis of breast carcinoma. Cancer 1996;77:2064‐9.