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Radiotherapy as a cisplatin‐sensitizer in a resistant ovarian carcinoma cell line
Author(s) -
Silver David F.,
Wheeless Clifford R.,
Dubin Norman H.
Publication year - 1996
Publication title -
cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.052
H-Index - 304
eISSN - 1097-0142
pISSN - 0008-543X
DOI - 10.1002/(sici)1097-0142(19960501)77:9<1850::aid-cncr14>3.0.co;2-z
Subject(s) - cisplatin , hemocytometer , medicine , radiation therapy , ovarian carcinoma , carcinoma , oncology , ovarian cancer , chemotherapy , ovary , cell culture , radiosensitivity , cancer research , pathology , cancer , biology , genetics
Abstract BACKGROUND Stage III ovarian carcinoma has shown resistance to adjuvant chemotherapy following surgical cytoreduction. With recurrence of ovarian carcinoma, cell lines may develop resistance to previously used chemotherapy. This contributes to the fact that survival rates for patients with ovarian carcinoma have not been dramatically improved in decades. The objective of this study is to evaluate radiotherapy as a cisplatin‐sensitizer in a cisplatin‐resistant ovarian carcinoma cell line. METHODS In vitro OVCAR‐3 human ovarian carcinoma cells were irradiated with external beam radiation (XRT) at doses of 500, 1,500, and 4,500 centigray (cGy) in a single fractionation. Twelve hours after XRT, cells were treated with a dose of cisplatin for 2 hours (0, 1, 3, 9, 30, and 90 ug/mL). Cell attachment was determined by cell counts using a hemocytometer under phase‐contrast microscopy. Analysis of variance followed by the Student Newman Keuls Test were used for statistical analysis. RESULTS Dose‐response curves demonstrate the results of this study as follows: (1) XRT has a significant direct effect on cell attachment of OVCAR‐3 cells in a dose‐response relationship. (2) cisplatin has no effect on cell attachment in the absence of XRT. (3) When cells are exposed to XRT, cisplatin demonstrates a dose‐response effect on cell attachment with a dose of XRT as low as 500 Gy. CONCLUSIONS This in vitro study suggests that XRT sensitizes cisplatin‐resistant OVCAR‐3 to cisplatin. This occurred with doses of radiation low enough to suggest a potential clinical role in treating resistant ovarian carcinoma. Cancer 1996;77:1850‐3.