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Crystallization and preliminary X‐ray analysis of the Escherichia coli replication terminator protein complexed with DNA
Author(s) -
Kamada Katsuhiko,
Ohsumi Katsufumi,
Horiuchi Takashi,
Shimamoto Nobuo,
Morikawa Kosuke
Publication year - 1996
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/(sici)1097-0134(199603)24:3<402::aid-prot14>3.0.co;2-q
Subject(s) - escherichia coli , dna replication , dna , crystallization , tetragonal crystal system , chemistry , terminator (solar) , crystallography , microbiology and biotechnology , resolution (logic) , biology , biophysics , biochemistry , crystal structure , gene , physics , ionosphere , organic chemistry , astronomy , artificial intelligence , computer science
Crystals of the Escherichia coli replication terminator protein (Tus) complexed with its binding site DNA were obtained by a microdialysis method using PEG 4000. They belong to the tetragonal space group P4 1 2 1 2 or P4 3 2 1 2 with the unit cell parameter: a = 68.1 Å, c = 230.7 Å and contain one protein‐DNA complex in an asymmetric unit. The native data set has been collected to 2.7 Å resolution.