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Identification of a decapeptide with the binding reactivity for tumor‐associated TAG72 antigen from a phage displayed library
Author(s) -
Gui Jin,
Moyana Terence,
Malcolm Bruce,
Xiang Jim
Publication year - 1996
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/(sici)1097-0134(199603)24:3<352::aid-prot8>3.0.co;2-i
Subject(s) - biopanning , antigen , phage display , microbiology and biotechnology , peptidomimetic , peptide library , blot , peptide , chemistry , biochemistry , biology , peptide sequence , genetics , gene
Peptide ligands for tumor‐associated TAG72 antigen were identified by screening a large, diverse decapeptide library expressed on the surface of filamentous phages. Fifty‐eight clones of phages were selected from the eluates after the third round of biopanning and their DNA inserts were sequenced. A dominant decapeptide HYVSIELPDH (14/58) was found with the binding reactivity for TAG72 antigen in the TAG72‐binding ELISA and Western dot blotting. It also showed a preferential binding to colonic adenocarcinomatous cells expressing the TAG72 antigen in the histochemical study. Therefore, this anti‐TAG72 decapeptide may be useful in serving as the starting point with regard to further designing peptidomimetics for potential pharmaceuticals.