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Crystallization and preliminary X‐ray analysis of volvatoxin A2 from Volvariella volvacea
Author(s) -
Lin SuChang,
Lin JungYaw,
Liaw YenChywan
Publication year - 1996
Publication title -
proteins: structure, function, and bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.699
H-Index - 191
eISSN - 1097-0134
pISSN - 0887-3585
DOI - 10.1002/(sici)1097-0134(199601)24:1<141::aid-prot11>3.0.co;2-o
Subject(s) - monoclinic crystal system , crystallization , volvariella volvacea , polyethylene glycol , mushroom , solvent , ammonium sulfate , molecule , chemistry , sodium azide , crystallography , nuclear chemistry , organic chemistry , food science
Volvatoxin A2, an ion channel disturbed cardiotoxic and hemolytic protein from the edible mushroom, Volvarilla volvacea , has been crystallized by the vapor diffusion method using polyethylene glycol 4000 and ammonium sulfate in sodium acetate buffer pH 4.6. The best crystals belong to the monoclinic space group C 2 with unit cell dimensions a = 155.25 Å, b = 58.06 Å, c = 116.92 Å, and β = 119.5°. These crystals diffract to at least 2.2 Å and there are four molecules of molecular weight 24 kDa per asymmetric unit with a solvent content of 48%.