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In vitro cadmium removal from human serum by Cibacron Blue F3GA–thionein‐complex conjugated affinity membranes
Author(s) -
Denizli Fatma,
Denizli Adil,
Yakup Arıca M
Publication year - 2000
Publication title -
polymer international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.592
H-Index - 105
eISSN - 1097-0126
pISSN - 0959-8103
DOI - 10.1002/(sici)1097-0126(200003)49:3<302::aid-pi366>3.0.co;2-r
Subject(s) - membrane , chemistry , adsorption , cadmium , methacrylate , chromatography , microporous material , langmuir adsorption model , polymer chemistry , nuclear chemistry , organic chemistry , polymer , copolymer , biochemistry
A new membrane affinity biosorbent carrying thionein has been developed for selective removal of cadmium ions from human serum. Microporous poly(2‐hydroxyethyl methacrylate) (pHEMA) membranes were prepared by photopolymerization of HEMA. The pseudo dye ligand Cibacron Blue F3GA (CB) was covalently immobilized on the pHEMA membranes. Then, the cysteine‐rich metallopeptide thionein was conjugated onto the CB‐immobilized membrane. The maximum amounts of CB immobilized and thionein conjugated on the membranes were 1.07 µmol cm −2 and 0.92 µmol cm −2 , respectively. The hydrophilic pHEMA membrane had a swelling ratio of 58% (w/w) with a contact angle of 45.8 °. CB‐immobilized and CB‐immobilized–thionein‐conjugated membranes were used in the Cd(II) removal studies. Cd(II) ion adsorption appeared to reach equilibrium within 30 min and to follow a typical Langmuir adsorption isotherm. The maximum capacity ( q m ) of the CB‐immobilized membranes was 0.203 (µmol Cd(II)) cm −2 membrane and increased to 1.48 (µmol Cd(II)) cm −2 upon CB–thionein‐complex conjugation. The pHEMA membranes retained their cadmium adsorption capacity even after 10 cycles of repeated use. © 2000 Society of Chemical Industry