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Gliadin nanoparticles: formation, all‐ trans ‐retinoic acid entrapment and release, size optimization
Author(s) -
Duclairoir Cécile,
Irache Juan Manuel,
Nakache Evelyne,
Orecchioni AnneMarie,
Chabenat Christiane,
Popineau Yves
Publication year - 1999
Publication title -
polymer international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.592
H-Index - 105
eISSN - 1097-0126
pISSN - 0959-8103
DOI - 10.1002/(sici)1097-0126(199904)48:4<327::aid-pi165>3.0.co;2-y
Subject(s) - solubility , gliadin , particle size , solvent , aqueous solution , nanoparticle , chemistry , chemical engineering , ionic strength , chromatography , materials science , organic chemistry , biochemistry , gluten , engineering
Gliadin nanoparticles were prepared by a desolvatation method. Theyshowed good stability during several weeks in PBS or aqueous medium.Assayed as carriers for all‐ trans ‐retinoic acid(RA), they have a quite good entrapment efficiency: about75% of added drug at 60 μg• (mg gliadin) −1 and a payload of 76.4 μg• (mggliadin) −1 nanoparticles. A rapid release of20% of the drug after 15 min in sink conditions and adiffusion process in a second step are observed. According to the nature of vegetal proteins, the size control ofthe nanoparticles may be reached by varying the nature of thesolvent, the pH and the ionic strength. Because gliadin is poorlysolubilized in aqueous solutions, the first method was chosen. Inorder to quantify more precisely the solvent effect, the sizediameter was optimized through a solubility parameter study. Thesmallest size was reached for protein solubility solvent equal tothat of gliadin. Size differences were observed with respect to theprocess steps; the diameter obtained after nanoprecipitationfluctuates with time. © 1999 Society of Chemical Industry