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Evaluation of in vitro stability of small unilamellar vesicles coated with collagen and chitosan
Author(s) -
Shi XiangYang,
Sun CaoMin,
Wu ShiKang
Publication year - 1999
Publication title -
polymer international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.592
H-Index - 105
eISSN - 1097-0126
pISSN - 0959-8103
DOI - 10.1002/(sici)1097-0126(199903)48:3<212::aid-pi127>3.0.co;2-8
Subject(s) - chitosan , liposome , membrane , vesicle , phosphatidylcholine , biophysics , membrane fluidity , permeability (electromagnetism) , fluorescence anisotropy , fluorescence , chemistry , in vitro , materials science , chromatography , phospholipid , biochemistry , physics , quantum mechanics , biology
To improve the in vitro stability of small unilamellarvesicles (SUV), the permeability of SUV, made from soyabeanphosphatidylcholine (PC) and coated with collagen andchitosan, was studied using 5(6)‐carboxyfluorescein(5(6)‐CF) as a fluorescence probe. Theresults showed that the coating with a collagen/PC weight ratioof 2, or a chitosan/PC weight ratio of 8, significantly decreasedthe permeability of liposomal membranes. In addition, thefluorescence polarization method was used to study the influence of acoating with collagen and chitosan at the above ratios on thefluidity of liposomal membranes, employing an intramolecular chargetransfer compound, a 3‐methoxy‐4′‐ N , N ‐dimethylaminoflavone derivative (DMMF),as a fluorescence probe. The fluidity of a liposomal membrane coatedwith either of the two macromolecules showed no obvious changes,indicating that SUV coated with collagen and chitosan at appropriateweight ratios, can significantly improve the in vitro stability of liposomal membranes without disturbing their fluidity. © 1999 Society of Chemical Industry