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Enzyme‐based bioartificial polymeric materials: the α‐amylase–poly(vinyl alcohol) system
Author(s) -
Cristallini Caterina,
Lazzeri Luigi,
Cascone Maria Grazia,
Polacco Giovanni,
Lupinacci Domenico,
Barbani Niccoletta
Publication year - 1997
Publication title -
polymer international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.592
H-Index - 105
eISSN - 1097-0126
pISSN - 0959-8103
DOI - 10.1002/(sici)1097-0126(199712)44:4<510::aid-pi884>3.0.co;2-0
Subject(s) - vinyl alcohol , crystallinity , materials science , thermal stability , self healing hydrogels , polymer , polymer chemistry , chemical engineering , immobilized enzyme , enzyme , chemistry , organic chemistry , composite material , engineering
The possibility of realizing bioartificial polymeric materials from blends of the enzyme α‐amylase (EC 3.2.1.1) and poly(vinyl alcohol), in the form of film and hydrogel, was investigated. The interactions at molecular level between the enzyme and the synthetic polymer have been studied by calorimetry, X‐ray diffractometry and morphological analysis. A certain degree of interaction through which the α‐amylase molecules influence the crystallinity of the poly(vinyl alcohol) was observed. The thermal stability of the enzyme in the blends was enhanced, while its biological activity did not vary significantly. In addition, hydrogels prepared by a freeze–thawing method can be considered suitable for manufacturing delivery systems capable of releasing enzymes which maintain their full biological activity. ©1997 SCI