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An RNA‐binding protein homologue that promotes sporulation‐specific gene expression in Saccharomyces cerevisiae
Author(s) -
Soushko Maria,
Mitchell Aaron P.
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(200005)16:7<631::aid-yea559>3.0.co;2-u
Subject(s) - biology , gene , gene expression , genetics , meiosis , rna , regulation of gene expression , saccharomyces cerevisiae , rna binding protein , microbiology and biotechnology
Prior studies have shown that S. cerevisiae rim4 mutations cause reduced expression of a sporulation‐specific reporter gene and sporulation. We report here that RIM4 (ORF YHL024W) is a putative RNA‐binding protein of the RRM class that is expressed at elevated levels early in meiosis. Mutations in the two RRMs reduce or abolish sporulation and, in some cases, cause reduced Rim4p expression. RIM4 is required for expression of early and middle sporulation‐specific genes. Unlike other meiotic regulatory genes, RIM4 is required for full gene activation through both the Ime1p and Ime2p pathways. The rim4Δ defect in activation by Ime2p is suppressed by a hyperactive Ime2p derivative. These observations argue that Rim4p may act upstream of Ime2p, perhaps in a nutritional sensing pathway. Copyright © 2000 John Wiley & Sons, Ltd.