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Sequence and organization analyses of a Zygosaccharomyces rouxii DNA fragment containing the HIS3 gene
Author(s) -
Sychrova Hana,
Braun Valerie,
Souciet JeanLuc
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(200005)16:7<581::aid-yea545>3.0.co;2-4
Subject(s) - biology , gene , genbank , genetics , complementation , nucleic acid sequence , saccharomyces cerevisiae , dna , genomic library , microbiology and biotechnology , genomic dna , open reading frame , genomic organization , accession number (library science) , mutant , peptide sequence , genome
The nucleotide sequence of a 3.4 kb fragment containing the HIS3 gene of the osmotolerant yeast Zygosaccharomyces rouxii has been determined. The fragment was cloned from a Z. rouxii genomic DNA library by complementation of a Saccharomyces cerevisiae his3 mutant strain. The sequenced DNA fragment contained three open reading frames; the middle one (678 bp long, predicting a protein of 226 amino acids) shared a high degree of similarity with HIS3 genes of other yeast species. In the promoter region of the putative ZrHIS3 gene, a T c element required for constitutive transcription was found. The GenBank Accession No. of the sequenced DNA region is Y18561. Copyright © 2000 John Wiley & Sons, Ltd.