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Molecular cloning of the CRM1 gene from Candida albicans
Author(s) -
Raymond Martine,
Dignard Daniel,
Alarco AnneMarie,
Clark Karen L.,
Weber Sandra,
Whiteway Malcolm,
Leberer Ekkehard,
Thomas David Y.
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(200004)16:6<531::aid-yea543>3.0.co;2-j
Subject(s) - biology , genetics , gene , genbank , saccharomyces cerevisiae , nucleic acid sequence , locus (genetics) , schizosaccharomyces pombe , homology (biology) , microbiology and biotechnology
In a screen for Candida albicans genes capable of supressing a ste20 Δ mutation in Saccharomyces cerevisiae , a homologue of the exportin‐encoding gene CRM1 was isolated. The CaCRM1 gene codes for a protein of 1079 amino acids with a predicted molecular weight of 124 029 and isoelectric point of 5.04. Crm1p from C. albicans displays significant amino acid sequence homology with Crm1p from Saccharomyces cerevisiae (65% identity, 74% similarity), Schizosaccharomyces pombe (55% identity, 66% similarity), Caenorhabditis elegans (45% identity, 57% similarity), and Homo sapiens (48% identity, 59% similarity). Interestingly, CaCRM1 encodes a threonine rather than a cysteine at position 533 in the conserved central region, suggesting that CaCrm1p is leptomycin B‐insensitive, like S. cerevisiae Crm1p. CaCRM1 on a high copy vector can complement a thermosensitive allele of CRM1 ( xpo1‐1 ) in S. cerevisiae , showing that CaCrm1p and S. cerevisiae Crm1p are functionally conserved. Southern blot analysis suggests that CaCRM1 is present at a single locus within the C. albicans genome. The nucleotide sequence of the CaCRM1 gene has been deposited at GenBank under Accession No. AF178855. Copyright © 2000 John Wiley & Sons, Ltd.