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Glycine metabolism in Candida albicans : characterization of the serine hydroxymethyltransferase ( SHM1, SHM2 ) and threonine aldolase ( GLY1 ) genes
Author(s) -
McNeil J. Bryan,
Flynn Jennifer,
Tsao Nora,
Monschau Nicole,
Stahmann K.Peter,
Haynes Robert H.,
McIntosh Evan M.,
Pearlman Ronald E.
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(20000130)16:2<167::aid-yea519>3.0.co;2-1
Subject(s) - biology , serine hydroxymethyltransferase , biochemistry , threonine , candida albicans , serine , aldolase a , gene , corpus albicans , glycine , amino acid , microbiology and biotechnology , genetics , yeast , enzyme
Genes encoding the mitochondrial ( SHM1 ) and cytosolic (SHM2 ) serine hydroxymethyltransferases, and the L ‐threonine aldolase gene ( GLY1 ) from Candida albicans were cloned and sequenced. All three genes are involved in glycine metabolism. The C. albicans Shm1 protein is 82% identical to that from Saccharomyces cerevisiae and 56% identical to that from Homo sapiens . The corresponding identities for the Shm2 proteins are 68% and 53%. The Gly1 protein shares significant identity with the S. cerevisiae L ‐threonine aldolase (55%) and also with threonine aldolases from Aeromonas jandiae (36%) and Escherichia coli (36%). Genetic ablation experiments show that GLY1 is a non‐essential gene in C. albicans and that L ‐threonine aldolase plays a lesser role in glycine metabolism than it does in S. cerevisiae . GenBank Accession Nos of the C. albicans SHM1 and SHM2 are AF009965 and AF009966, respectively. Accession No. for C. albicans GLY1 is AF009967. Copyright © 2000 John Wiley & Sons, Ltd.