Premium
Isolation and sequence of the HOG1 homologue from Debaryomyces hansenii by complementation of the hog1 Δ strain of Saccharomyces cerevisiae
Author(s) -
Bansal Parmil K.,
Mondal Alok K.
Publication year - 2000
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(20000115)16:1<81::aid-yea510>3.0.co;2-i
Subject(s) - biology , saccharomyces cerevisiae , complementation , genbank , genetics , open reading frame , gene , debaryomyces hansenii , peptide sequence , yeast , phenotype
The HOG1 gene encodes a MAP kinase that plays an essential role in maintaining water homeostasis in the yeast Saccharomyces cerevisiae . A gene homologous to S. cerevisiae HOG1 has been isolated from a highly salt‐tolerant yeast, Debaryomyces hansenii , by phenotypic complementation. DNA sequencing of the clone revealed the presence of an open reading frame encoding a protein 387 amino acids long. The deduced amino acid sequence showed very high similarity with homologous genes identified from S. cerevisiae , Candida albicans and Zygosaccharomyces rouxii . In addition, it has also TGY motif characteristics of hyperosmolarity‐activated MAP kinases. The Genbank Accession No. of this sequence is AF185278. Copyright © 2000 John Wiley & Sons, Ltd.