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Transcription of some PHO genes in Saccharomyces cerevisiae is regulated by Spt7p
Author(s) -
Nishimura Ken,
Yasumura Kyoko,
Igarashi Kazuei,
Harashima Satoshi,
Kakinuma Yoshimi
Publication year - 1999
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199912)15:16<1711::aid-yea497>3.0.co;2-8
Subject(s) - regulon , biology , gene , mutant , saccharomyces cerevisiae , transcription factor , yeast , northern blot , transcription (linguistics) , genetics , gene expression , microbiology and biotechnology , linguistics , philosophy
Spt7p is a new global transcription factor in Saccharomyces cerevisiae (Gansheroff et al. , 1995). We report here that the activities of high affinity phosphate transport and acid phosphatase in particular were decreased in a spt7 null mutant. Northern blot experiments revealed that transcription of the PHO84 and PHO5 genes was impaired in this mutant; expression of the PHO regulatory genes, PHO4 and PHO2 , was normal. Spt7p is thus linked with expression of several structural genes of the PHO regulon in yeast. Copyright © 1999 John Wiley & Sons, Ltd.