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Transformation of Candida albicans by electroporation
Author(s) -
De Backer Marianne D.,
Maes Dirk,
Vandoninck Sandy,
Logghe Marc,
Contreras Roland,
Luyten Walter H. M. L.
Publication year - 1999
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199911)15:15<1609::aid-yea485>3.0.co;2-y
Subject(s) - electroporation , transformation (genetics) , transformation efficiency , candida albicans , plasmid , biology , spheroplast , dna , protoplast , microbiology and biotechnology , computational biology , genetics , gene , escherichia coli , agrobacterium
In contrast to a variety of other yeasts, Candida albicans has proved difficult to transform with high efficiency. Lithium acetate transformation is fast and simple but provides a very low efficiency of DNA transfer (50–100 transformants/µg DNA), while spheroplast transformation, although more efficient (∼300 transformants/µg integrative DNA and 10 3 –10 4 transformants/µg replicative DNA), is complicated and time‐consuming. In this study we applied various yeast transformation techniques to C. albicans and selected an electroporation procedure for further optimization. Transformation efficiencies of up to 300 transformants/µg were obtained for an integrative plasmid and up to 4500 transformants/µg for a CARS‐carrying plasmid. This reasonably high transformation efficiency, combined with the ease and speed of electroporation in comparison to alternative techniques, make it the preferred method for transformation of C. albicans. Copyright © 1999 John Wiley & Sons, Ltd.