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Molecular cloning and sequence analysis of Zygosaccharomyces rouxii ADE2 gene encoding a phosphoribosyl‐aminoimidazole carboxylase
Author(s) -
Sychrova Hana,
Braun Valerie,
Souciet JeanLuc
Publication year - 1999
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19990930)15:13<1399::aid-yea464>3.0.co;2-r
Subject(s) - biology , complementation , nucleic acid sequence , gene , homology (biology) , open reading frame , genetics , molecular cloning , microbiology and biotechnology , peptide sequence , sequence analysis , mutant , saccharomyces cerevisiae , accession number (library science) , yeast , structural gene , genbank
The nucleotide sequence of a 2·8 kb fragment containing the ADE2 gene of the osmotolerant yeast Zygosaccharomyces rouxii has been determined. The gene was cloned from a Z. rouxii genomic DNA library by complementation of the Saccharomyces cerevisae ade2 mutant strain. The sequenced DNA fragment contains a 1710 bp open reading frame predicting a protein of 570 amino acids. The deduced amino acid sequence shares a high degree of homology with Ade2p homologues in five other yeast species. The Gene Bank Accession No. of Z‐ADE2 is AJ133372. Copyright © 1999 John Wiley & Sons, Ltd.