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ade9 is an allele of SER1 and plays an indirect role in purine biosynthesis
Author(s) -
Buc Petra S.,
Rolfes Ronda J.
Publication year - 1999
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19990930)15:13<1347::aid-yea461>3.0.co;2-a
Subject(s) - biology , biosynthesis , serine , purine metabolism , purine , locus (genetics) , genetics , allele , biochemistry , saccharomyces cerevisiae , phenotype , gene , enzyme
In this study we demonstrate that ade9 plays an indirect role in purine biosynthesis as a non‐functional allele of SER1 in Saccharomyces cerevisiae . The SER1 locus, encoding 3‐phosphoserine aminotransferase required for serine biosynthesis, is located on chromosome XV and resides approximately where ade9 had previously been mapped genetically. A minimal functional construct of SER1 is necessary and sufficient to complement both the adenine‐ and serine‐requiring phenotypes of ade9 strains. In addition, adequate exogenous serine levels mask the adenine phenotype of ade9 . A disruption of SER1 behaves in the same manner phenotypically as the original ade9 strain. Therefore, ade9 can be more accurately described as an allele of SER1 . Copyright © 1999 John Wiley & Sons, Ltd.