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Cell cycle regulation of a DNA ligase‐encoding gene ( CaLIG4 ) from Candida albicans
Author(s) -
Andaluz E.,
Ciudad A.,
Rubio Coque J.,
Calderone R.,
Larriba G.
Publication year - 1999
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(19990915)15:12<1199::aid-yea447>3.0.co;2-s
Subject(s) - biology , dna ligase , saccharomyces cerevisiae , dna replication , gene , microbiology and biotechnology , transcription (linguistics) , candida albicans , dna , genetics , linguistics , philosophy
A DNA ligase ( CaLIG4 ) (formerly CaCDC9 ) of the human pathogen, Candida albicans , has been characterized. The encoded protein displayed a significant similarity to ligase IV from both Saccharomyces cerevisiae and humans. In addition, whereas CaLIG4 did not complement a S. cerevisiae cdc9 mutant, it re‐established non‐homologous end‐joining of DNA double‐strand breaks in a S. cerevisiae lig4 deletant. CaLIG4 was assigned to chromosome 2. Several cis ‐acting effector sequences were identified in the promoter region of the CaLIG4 , including the DNA sequence element ACGNG, which is required for periodic transcription of several DNA‐replicating genes in S. cerevisiae . The level of transcription of CaLIG4 in C. albicans varies during the yeast cell cycle. Newly formed cells contained basal levels of transcript which increased to a maximum level when cells were in late G 1 . Thereafter, levels of transcript dropped as DNA replication was initiated. Our results suggest that CaLIG4 may perform an important role during the mitotic cycle of C. albicans . Copyright © 1999 John Wiley & Sons, Ltd.