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Molecular cloning of homologs of RAS and RHO1 genes from Cryptococcus neoformans
Author(s) -
Tanaka Kenichi,
Nambu Hirohide,
Katoh Yoshiaki,
Kai Mayumi,
Hidaka Yusuke
Publication year - 1999
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199908)15:11<1133::aid-yea438>3.0.co;2-n
Subject(s) - biology , schizosaccharomyces pombe , gene , peptide sequence , amino acid , cryptococcus neoformans , genetics , saccharomyces cerevisiae , nucleic acid sequence , biochemistry , candida albicans , cloning (programming) , microbiology and biotechnology , computer science , programming language
We cloned and sequenced homologs of RAS ( CnRAS ) and RHO1 ( CnRHO1 ) genes from Cryptococcus neoformans . The proteins encoded by the CnRAS and CnRHO1 genes contained 216 and 197 amino acids, respectively. The deduced amino acid sequence of the CnRAS gene shared a high degree of sequence identity with the Ras proteins in other fungal species: Coprinus cinereus (76%), Lentinula edodes (74%), Saccharomyces cerevisiae RAS2 (72%), and Schizosaccharomyces pombe (68%). The deduced amino acid sequence of the CnRHO1 gene shared a high degree of sequence identity with the Rho1 proteins in other fungal species: Candida albicans (78%), S. pombe (77%) and S. cerevisiae (76%). The deduced proteins contained GTP‐binding and GTP‐hydrolysis domains, and the prenylation site that are conserved among the small G protein superfamily. The synthetic peptides that contained the C‐terminal amino acid sequence of the Cn Ras and Cn Rho1 proteins were geranylgeranylated. The DDBJ/EMBL/GenBank Accession Numbers of the CnRAS and CnRHO1 genes are AB017640 and AB017639, respectively. Copyright © 1999 John Wiley & Sons, Ltd.