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Schizosaccharomyces pombe homologue of glutathione peroxidase, which does not contain selenocysteine, is induced by several stresses and works as an antioxidant
Author(s) -
Yamada Kenichiro,
Nakagawa Chiaki W.,
Mutoh Norihiro
Publication year - 1999
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/(sici)1097-0061(199908)15:11<1125::aid-yea442>3.0.co;2-z
Subject(s) - gpx1 , schizosaccharomyces pombe , biology , selenoprotein , selenocysteine , gene , microbiology and biotechnology , repressor lexa , genetics , biochemistry , glutathione peroxidase , gene expression , catalase , oxidative stress , enzyme , cysteine , saccharomyces cerevisiae , repressor
We have cloned a gene of Schizosaccharomyces pombe homologues to the glutathione peroxidase gene. The cloned gene, named gpx1 + , encoded a protein that was 158 amino acids in length and had a molecular mass of 18 kDa. The gpx1 + gene is homologous with many glutathione peroxidase genes but the selenocysteine codon (UGA) position of mammalian genes is a cysteine codon (UGU) in S. pombe . gpx1 + mRNA was induced by various stresses, including oxidative stress, osmostress and heat stress. These stresses activate the Wis1–Sty1/Spc1 MAP kinase cascade in S. pombe . Transcriptional factors Atf1 and Pap1 are under the control of this MAP kinase. In the disruption of the atf1 + gene, gpx1 + was not transcribed or induced. However, the expression of gpx1 + was not affected by the disruption of the pap1 + gene. These results indicated that gpx1 + was under the control of transcription factor Atf1. Catalase can detoxicate H 2 O 2 in the same way as GPx and the disruptant of the catalase gene of S. pombe is hypersensitive to H 2 O 2 . The catalase gene disruptant of S. pombe harbouring multicopy plasmid containing gpx1 + restored the hypersensitivity to H 2 O 2 of the catalase gene disruptant. These results suggest that Gpx1 acts as a scavenger of H 2 O 2 in vivo. The nucleotide sequence reported in this paper has been submitted to DDBJ/EMBL/GenBank, with Accession No. AB012395. Copyright © 1999 John Wiley & Sons, Ltd.